Products & Services

iCell® Motor Neurons

Moving Research Forward with New Model Systems

Innervation of muscle fibers by spinal motor neurons is essential for controlling muscle contraction and ultimately coordinated movement. Neurodegenerative diseases, such as ALS and SMA, are a result of the progressive degeneration of motor neurons that leads to an uncoupling of this process. In order to provide researchers with a model system for understanding basic motor neuron biology and to develop treatments for these diseases, CDI offers iCell® Motor Neurons. iCell Motor Neurons are a highly pure population of human spinal motor neurons derived from induced pluripotent stem (iPS) cells with the following features:

  • Fully differentiated, ≥87% (β-III tubulin) pure population of primarily cholinergic human neurons (≥55% Isl1/2)
  • Display typical neuronal morphology with branching neurites and remain viable for an extended culture period (≥14 days)
  • Express markers characteristic of motor neurons
  • Available in commercial quantities
  • Easy to use: Simply thaw and plate

These cells provide a reliable source of human neurons suitable for use within:

  • Basic and translational research: Studying neurodegenerative diseases like ALS & SMA, myelination/demyelination, and neurogenesis
  • Drug discovery: For diseases such as ALS and SMA
  • Bioengineering: Development of neuromuscular junction models
  • Applied markets: BoNT testing
  • Toxicology and safety pharma: For testing chemical warfare agents, effects of chemical pesticides, etc.

Figure 1. Flow Cytometry Analysis
Flow cytometry data verify a highly pure (Tuj positive), fully differentiated (Nestin negative) motor neuron (Isl1/2) population.

Figure 2. Morphology
The cells display typical morphology, developing branched networks within 1 DIV (Panel A), expanding beyond 7 DIV (Panel B) and forming ganglionic structures near 14 DIV (Panel C).

Figure 3. Immunocytochemical Analysis
iCell Motor Neurons were stained at 14 DIV for characteristic neuronal and motor neuron markers.

Figure 4. Gene Expression Analysis
qPCR was performed at 14 DIV to assess the relative expression of neuronal and motor neuron markers within two different lots of iCell Motor Neurons, spinal cord, and iPSCs.

  • Ordering Information
    Catalog # Description Quantity
    MNC-301-030-000.5-PT iCell Motor Neurons Kit

    Each kit contains:

    • Cryopreserved iCell Motor Neurons
    • 100 ml iCell Neural Base Medium 1
    • 2 ml  iCell Neural Supplement A
    • 1 ml iCell Nervous System Supplement
    • User’s Guide
    ≥1.6 x 106
    Viable Cells/Vial
    Request Quote
    R1049 iCell Motor Neurons Kit, 01279

    Each kit contains:

    • Cryopreserved iCell Motor Neurons
    • 100 ml iCell Neural Base Medium 1
    • 2 ml iCell Neural Supplement A
    • 1 ml iCell Nervous System Supplement
    • User's Guide
    ≥3.0 x 106
    Viable Cells/Vial
    Request Quote
    M1010 iCell Neural Base Medium 1

    Neural maintenance medium, serum-free

    100 ml (1X) Request Quote
    M1031 iCell Nervous System Supplement

    Growth factor supplement for neuronal cell types

    1 ml Request Quote
    M1032 iCell Neural Supplement A

    Neuronal medium supplement, serum-free

    2 ml (1X) Request Quote