Applications

Regenerative Medicine

Novel Regenerative Medicine Approaches

To date, most human in vivo cell clinical and tissue engineering studies have employed adult stem and progenitor cells. However, initial clinical trials utilizing pluripotent stem cells are underway, and these cells may eventually emerge as a more tractable source of human material. iCell® and MyCell® products are being employed in a number of regenerative medicine applications:

  • Regenerative medicine compound screening: Due to their biological relevance and compatibility with high-throughput screening paradigms, iCell and MyCell products enable identification of novel regenerative compounds
  • Tissue engineering: iCell and MyCell products can be used to bioengineer implantable devices, reconstitute decellularized organs, and manufacture organs using 3D bioprinting
  • Allogeneic and autologous cell applications: iPS cell technology may one day be used for allogeneic and autologous cell applications with the added benefit of being able to introduce a genetically corrected disease mutation in this context

CDI’s cellular reprogramming technologies, intellectual property portfolio, and manufacturing capabilities have broad applicability to the design, development, and manufacturing of future cell-based therapies. Translation of these therapeutic approaches to the clinic will require HLA-matched banks of iPS cells generated under good manufacturing practice (GMP) conditions, a project currently underway in a CDI partnership with Waisman Biomanufacturing.

Contact us to learn more about these exciting advances.

Advanced Cardiomyocyte Cell Culture

Advanced cell culture techniques including 3D spheroids, micropatterned co-culture, bioengineered and flow-based systems, and bioprinting offer the potential to better mimic in vivo tissue structure and function.

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Advanced Cardiomyocyte Cell Culture

Discovery, Regenerative Medicine, Toxicity

Advanced cell culture techniques including 3D spheroids, micropatterned co-culture, bioengineered and flow-based systems, and bioprinting offer the potential to better mimic in vivo tissue structure and function. CDI’s cardiomyocytes are amenable to these culture techniques as pure cell populations or in co-culture with other cell types, such as CDI’s iCell Endothelial Cells.

  1. Carlson C, Einhorn S, et al. (2013) Applications Development at CDI: Improving Workflows, Pushing Biology, and Enabling Screening. Poster Presentation, Cellular Dynamics User Group Meeting.
  2. Rao C, Prodromakis T, et al. (2013)  The Effect of Microgrooved Culture Substrates on Calcium Cycling of Cardiac Myocytes Derived from Human Induced Pluripotent Stem Cells. Biomaterials 34(10):2399-411.
  3. iCell Cardiomyocytes – iCell Endothelial Cells Co-culture. Contact Technical Support for more information.

Genetic Manipulation of Cardiomyocytes

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function.

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Genetic Manipulation of Cardiomyocytes

Discovery, Regenerative Medicine, Toxicity

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function. CDI’s cardiomyocytes are amenable to various genetic manipulation techniques including transfection, transduction, siRNA, and reporter vector expression.

Transfection and Transduction:

  1. iCell Products: Applying Transfection Technologies to Create Novel Screening Models. Cellular Dynamics Application Note.
  2. Robers M and Jarecki B. (2014) Efficiently Build Relevant In Vitro Models Using Human Stem Cell-derived Tissue Cells, High Performance Transfection and Novel Multiplexed Reporter Techniques. Promega/Cellular Dynamics Webinar.
  3. Anson B. (2015) Building Richer Assays: iPSC-derived Tissue Cells Are a Powerful Addition to the Biologist’s Tool Box. GEN 35(2).
  4. Traister A, Li M, et al. (2014) Integrin-linked Kinase Mediates Force Transduction in Cardiomyocytes by Modulating SERCA2a/PLN Function. Nature Comm 5:4533.
  5. Fine M, Lu F, et al. (2013) Human Induced Pluripotent Stem Cell-derived Cardiomyocytes for Studies of Cardiac Ion Transporters. Am J Physiol Cell Physiol 305(5):C481-91.

siRNA:

  1. iCell Products: Applying Transfection Technologies to Create Novel Screening Models. Cellular Dynamics Application Note.
  2. Traister A, Li M, et al. (2014) Integrin-linked Kinase Mediates Force Transduction in Cardiomyocytes by Modulating SERCA2a/PLN Function. Nature Comm 5:4533.
  3. Fine M, Lu F, et al. (2013) Human Induced Pluripotent Stem Cell-derived Cardiomyocytes for Studies of Cardiac Ion Transporters. Am J Physiol Cell Physiol 305(5):C481-91.

Reporter Vector Expression:

  1. Robers M and Jarecki B. (2014) Efficiently Build Relevant In Vitro Models Using Human Stem Cell-derived Tissue Cells, High Performance Transfection and Novel Multiplexed Reporter Techniques. Promega/Cellular Dynamics Webinar.

Bioengineering Cardiac Tissues

CDI's cardiomyocytes are being applied in research aimed to develop and test a bioengineered, implantable cardiac patch for the treatment of heart failure.

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Bioengineering Cardiac Tissues

Regenerative Medicine

CDI’s cardiomyocytes are being applied in research aimed to develop and test a bioengineered, implantable cardiac patch for the treatment of heart failure. Initial studies demonstrate that the cardiac patches beat spontaneously and synchronously, respond to electrical stimulation, exhibit typical morphology, and improve cardiac function in rats with chronic heart failure.

  1. Lancaster J. (2012) Development and Testing of a Cardiomyocyte Scaffold for the Treatment of Heart Failure. Cellular Dynamics User Group Meeting.

Advanced Neural Cell Culture

Advanced cell culture techniques including 3D spheroids, micropatterned co-culture, bioengineered and flow-based systems, and bioprinting offer the potential to better mimic in vivo tissue structure and function.

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Advanced Neural Cell Culture

Discovery, Regenerative Medicine, Toxicity

Advanced cell culture techniques including 3D spheroids, micropatterned co-culture, bioengineered and flow-based systems, and bioprinting offer the potential to better mimic in vivo tissue structure and function. CDI’s neurons are amenable to these culture techniques as pure cell populations or in co-culture with other cell types, such as CDI’s astrocytes.

  1. Carlson C, Wang J, et al. (2014) Characterization of an Isogenic Disease Model of Alzheimer’s Disease from Human iPSC-derived Neurons. Poster Presentation, Society for Neuroscience.
  2. DeLaura S, Fluri DA, et al. (2014) Human Neural Microtissues Derived from Induced Pluripotent Stem Cells for Toxicity Testing. Poster Presentation, Society for Neuroscience.

Genetic Manipulation of Neurons

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function.

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Genetic Manipulation of Neurons

Discovery, Regenerative Medicine, Toxicity

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function. CDI’s neurons and dopaneurons are amenable to various genetic manipulation techniques including:

  • Transfection
  • Transduction
  • siRNA
  • Reporter vector expression

Measuring Cardiac Progenitor Cell Proliferation and Differentiation

The ability to model human cell and organ developmental pathways is critical to understanding developmental toxicities and disease pathways, and to develop therapeutic strategies for tissue regeneration.

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Measuring Cardiac Progenitor Cell Proliferation and Differentiation

Discovery, Regenerative Medicine, Toxicity

The ability to model human cell and organ developmental pathways is critical to understanding developmental toxicities and disease pathways and to developing therapeutic strategies for tissue regeneration. CDI’s cardiac progenitor cells are multipotent cardiomyocyte precursor cells that exhibit robust and measurable proliferation and differentiation. Assays with these cells are being used in targeted and phenotypic screens to identify therapeutic candidates for cardiac regeneration.

Genetic Manipulation of Hepatocytes

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function.

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Genetic Manipulation of Hepatocytes

Discovery, Regenerative Medicine, Toxicity

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function. CDI has evaluated various genetic manipulation tools to enable the development of assays using its hepatocytes.

Advanced Hepatocyte Cell Culture

Advanced cell culture techniques including 3D spheroids, micropatterned co-culture, bioengineered and flow-based systems, and bioprinting offer the potential to better mimic in vivo tissue structure and function.

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Advanced Hepatocyte Cell Culture

Discovery, Regenerative Medicine, Toxicity

Advanced cell culture techniques including 3D spheroids, micropatterned co-culture, bioengineered and flow-based systems, and bioprinting offer the potential to better mimic in vivo tissue structure and function. CDI’s hepatocytes are amenable to these culture techniques as pure cell populations or in co-culture with other CDI cell types.

Vascular Tissue Bioengineering

Vascular networks supply organs with oxygen and nutrients, remove waste, and serve generally as the delivery network within the body.

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Vascular Tissue Bioengineering

Discovery, Regenerative Medicine, Toxicity

Vascular networks supply organs with oxygen and nutrients, remove waste, and serve generally as the delivery network within the body. Thus, any bio- or tissue engineering effort should include a vascular framework to support organ function. CDI’s endothelial cells have demonstrated functionality to reform vascular networks in decellularized organs to support de novo organ synthesis as a transplantable tissue for regenerative medicine approaches. In addition, CDI’s endothelial cells have formed complex vascular networks in static- and flow-based bioengineered vascular platforms.

Measuring Vasculogenesis

The ability to modulate vasculogenesis has utility in tissue engineering and repair as well as in oncology therapeutics development aimed at targeting angiogenesis.

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Measuring Vasculogenesis

Discovery, Regenerative Medicine

The ability to modulate vasculogenesis has utility in tissue engineering and repair as well as in oncology therapeutics development aimed at targeting angiogenesis. The processes of endothelial cell migration and invasion and vascular sprouting behavior can be measured using CDI’s endothelial cells using platforms including:

  1. Belair DG, Whisler JA, et al. (2014) Human Vascular Tissue Models Formed from Human Induced Pluripotent Stem Cell Derived Endothelial Cells. Stem Cell Rev. [Epub ahead of print]
  2. Belair D, Carlson C, et al. (2014) Label-free, Real-time Analysis of Endothelial Cell Morphogenesis Using iPSC-derived Endothelial Cells. Poster Presentation, AACR.

Measuring Neuronal Electrophysiology

The communication between neurons and between neurons and other cell types is accomplished through electrical signals.

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Measuring Neuronal Electrophysiology

Discovery, Regenerative Medicine, Toxicity

The communication between neurons and between neurons and other cell types is accomplished through electrical signals. CDI’s neurons exhibit biologically relevant electrical functions typical of primary human cortical neurons including evoked and spontaneous action potentials, inhibitory and excitatory post-synaptic currents, and ion channel pharmacology. These responses can be measured using platforms including:

Measuring Vascular Endothelial Cell Proliferation

The regulation of endothelial cell proliferation plays a fundamental role in vascular remodeling and angiogenesis in normal and pathological conditions.

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Measuring Vascular Endothelial Cell Proliferation

Discovery, Regenerative Medicine, Toxicity

The regulation of endothelial cell proliferation plays a fundamental role in vascular remodeling and angiogenesis in normal and pathological conditions. CDI’s endothelial cells exhibit a dose-dependent proliferation response to VEGF that is sensitive to inhibition by tyrphostin, a selective VEGF receptor inhibitor, as measured using the CellTiter-Glo Assay (Promega).

  1. Assaying Cell Proliferation. Cellular Dynamics Application Note.
  2. Belair D, Carlson C, et al. (2014) Label-free, Real-time Analysis of Endothelial Cell Morphogenesis Using iPSC-derived Endothelial Cells. Poster Presentation, AACR.

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